Ostrom Lab Publications


Original Articles
Book Chapters, Invited Publications and Review Articles

Original Articles

Bogard AS, Birg AV, Ostrom RS. Non-raft adenylyl cyclase 2 defines a cAMP signaling compartment that selectively regulates IL-6 expression in airway smooth muscle cells: differential regulation of gene expression by AC isoforms. Naunyn Schmiedebergs Arch Pharmacol. 387(4):329-39, 2014      PubMed

Cai W, Du A, Feng K, Zhao X, Qian L, Ostrom RS, Xu C.  Adenylyl Cyclase 6 Activation Negatively Regulates TLR4 Signaling through Lipid Raft-Mediated Endocytosis.  J Immunol, 191(12):6093-100, 2013.     PubMed

Conley JM, Brand CS, Bogard AS, Pratt EP, Xu R, Hockerman GH, Ostrom RS, Dessauer CW, Watts VJ.  Development of a high-throughput screening paradigm for the discovery of small-molecule modulators of adenylyl cyclase: identification of an adenylyl cyclase 2 inhibitor. J Pharmacol Exp Ther. 347(2):276-87, 2013.     PubMed

Amy S. Bogard, Piyatilake Adris and Rennolds S Ostrom. Adenylyl cyclase 2 (AC2) selectively couples to EP2 receptors while adenylyl cyclase 3 (AC3) is not receptor regulated in airway smooth muscle. J Pharmacol Exp Thera, 342(2):586-95, 2012.     PubMed     JPET2012MS.pdf

Amy S. Bogard, Congfeng Xu and Rennolds S Ostrom. Human bronchial smooth muscle cells express adenylyl cyclase isoforms 2, 4 and 6 in distinct membrane microdomains. J Pharmacol Exp Thera, 337(1): 209-17, 2011.    PubMed     JPET2011MS.pdf

Xiaoqiu Liu, Fengying Li, Shu Qiang Sun, Muthusamy Thangavel, Joseph Kaminsky, Louisa Balazs and Rennolds S Ostrom. Fibroblast specific expression of AC6 enhances ß-adrenergic and prostacyclin signaling and blunts bleomycin-induced pulmonary fibrosis.  Am J Physiol Lung Cell Mol Physiol, 298(6): L819-29, 2010.     PubMed

Muthusamy Thangavel, Xiaoqiu Liu, Shu Qiang Sun, Joseph Kaminsky and Rennolds S Ostrom. The C1 and C3 domains target human type 6 adenylyl cyclase to lipid rafts and caveolae.  Cell Signalling, 21:301-308, 2008. PubMed

Xiaoqiu Liu, Muthusamy Thangavel, Shu Qiang Sun, Joseph Kaminsky, Penden Mahautmr, Jeremiah Stitham, John Hwa and Rennolds S Ostrom. Adenylyl cyclase type 6 overexpression selectively enhances beta-adrenergic and prostacyclin receptor-mediated inhibition of cardiac fibroblast function because of colocalization in lipid rafts. Naunyn Schmiedebergs Arch Pharmacol, 377(4-6):359-69, 2007.  PubMed

Xiaoqiu Liu, Shu Qiang Sun, Aviv Hassid and Rennolds S Ostrom.  cAMP inhibits TGF-ß-stimulated collagen synthesis via inhibition of ERK1/2 and Smad signaling in rat cardiac fibroblasts.  Mol Pharmacol, 70:1992-2003, 2006.    PubMed

Liu X, Sun SQ, Ostrom RS. Fibrotic pulmonary fibroblasts show blunted inhibition by cAMP due to deficient CREB phosphorylation. J Pharmacol Exp Thera, 315(2):678-87, 2005 .    PubMed

Ostrom RS, Bundey RA, Insel PA. Nitric oxide inhibition of adenylyl cyclase type
6 activity is dependent upon lipid rafts and caveolin signaling complexes. J Biol Chem, 279(19): 19846-53, 2004.  PubMed

Liu X, Ostrom RS, Insel PA. Cyclic AMP-elevating agents and adenylyl cyclase over-expression promote an anti-fibrotic phenotype in pulmonary fibroblasts. Am J Physiol Cell Physiol, 286(5):C1089-99, 2004.   PubMed

Ostrom RS, Naugle JE, Hase M, Gregorian C, Swaney JS, Insel PA, Brunton LL, Meszaros JG. Angiotensin II enhances adenylyl cyclase signaling via Ca2+/calmodulin. Gq-Gs cross-talk regulates collagen production in cardiac fibroblasts. J Biol Chem, 278(27): 24461-8, 2003.   PubMed

Loomis WH, Namiki S, Ostrom RS, Insel PA, Junger WG. Hypertonic Stress Increases T Cell Interleukin-2 Expression through a Mechanism That Involves ATP Release, P2 Receptor, and p38 MAPK Activation, J Biol Chem, 278(7):4590-6, 2003.  PubMed

Ostrom RS, Liu X, Gregorian C, Drenan RM, Seasholtz TM, Insel PA. Compartmentation of adenylyl cyclase isoform expression and receptor coupling in aortic smooth muscle cells, Mol Pharmacol , 62:983-992, 2002.  PubMed

Zheng B, Ma Y-C, Ostrom RS, Lavoie C, Gill GN, Insel PA, Huang X-Y, Farquhar MG. RGS-PX1: A gap for G(s and a sorting nexin in vesicular trafficking, Science, 294:1939-1942, 2001. PubMed

Ostrom RS, Gregorian C, Drenan RM, Xiang Y, Regan JW, Insel PA. Receptor number and caveolar co-localization determine receptor coupling efficiency to adenylyl cyclase, J Biol Chem , 276(45):42063-9, 2001  PubMed

Ostrom RS, Gregorian C, Drenan RM, Gabot K, Rana BK, Insel PA. Key role for constitutive cyclooxygenase-2 of MDCK cells in basal signaling and response to released ATP, Am J Physiol: Cell Physiol, 281:C524-C531, 2001. PubMed

Ostrom RS, Gregorian C, Insel PA. Cellular release of and response to ATP as key determinants of the set-point of signal transduction pathways J Biol Chem, 275:11735-11739, 2000.  PubMed

Ostrom RS, Violin JD, Coleman S, Insel PA. Selective enhancement of ß-adrenergic receptor signaling by overexpression of adenylyl cyclase type 6: co-localization of receptor and adenylyl cyclase in caveolae of cardiac myocytes. Mol Pharmacol, 57:1075-1079, 2000.  PubMed

Book Chapters, Invited Publications and Review Articles

Rennolds S Ostrom. A two-pronged weapon in the fight against fibrosis. Focus on “Inhibition of Wnt/β-catenin signaling promotes epithelial differentiation of mesenchymal stem cells and repairs bleomycin-induced lung injury”. Am J Physiol Cell Physiol 307(3):C232-3, 2014.     PubMed

Rennolds S Ostrom.  A new molecular target for blunting organ fibrosis. Focus on “Secreted Frizzled-related protein 2 as a target in antifibrotic therapeutic intervention”. Am J Physiol Cell Physiol. 306(6):C527-82014.     PubMed

Rennolds S Ostrom, Amy S. Bogard, Robert Gros and Ross D. Feldman.  Choreographing the adenylyl cyclase signalosome: sorting out the partners and the steps. Naunyn-Schmiedebergs Arch Pharmacol, 385(1):5-12, 2012.   PubMed

Radomir M. Slominski, Russel J. Reiter, Natalia Schlabritz-Loutsevitch, Rennolds S Ostrom and Andrzej T. Slominski. Melatonin Membrane Receptors in Peripheral Tissues: Distribution and Functions. Mol Cell Endocrin, 351:152-166, 2012.   PubMed

Hemal H. Patel and Rennolds S Ostrom.  An orphan GPCR finds a home in the heart.  Am J Physiol Cell Physiol, 295(2):H479-81, 2008    PubMed

Rennolds S Ostrom and Xiaoqiu Liu. Detergent and detergent-free methods to define lipid rafts and caveolae.  Methods in Molecular Biology, vol. 400: Methods in Membrane Lipids.  A.M. Dopico, ed.  Humana Press Inc., Totowa, NJ, 2008.    PubMed

Rennolds S. Ostrom and Paul A. Insel. Methods for the study of signaling molecules in membrane lipid rafts and caveolae. Methods in Molecular Biology, vol. 332: Transmembrane Signaling Protocols, 2nd Edition.  Ali and Haribabu, ed.  Humana Press Inc., Totowa, NJ, 2006.    PubMed

Insel PA, Head BP, Ostrom RS, Patel HH, Swaney JS, Tang CM and Roth DM.  Caveolae and lipid rafts: G protein-coupled receptor signaling microdomains in cardiac myocytes.  Ann N Y Acad Sci, 1047:166-172, 2005.     PubMed

Ostrom RS. Caveolins muscle their way into the regulation of cell differentiation,
development, and function. Focus on “Muscle-specific interaction of caveolin isoforms: differential complex formation between caveolins in fibroblastic vs. muscle cells.”  Am J Physiol: Cell Physiol, 288:C507-C509, 2005.   PubMed

Ostrom RS, Insel PA.  The evolving role of lipid rafts and caveolae in G protein-coupled receptor signaling: Implications for molecular pharmacology.  Brit J Pharmacol, 143(2):235-45, 2004.   PubMed

Ostrom RS and Insel PA. Forskolin as a tool for examining adenylyl cyclase expression, regulation and G protein signaling. Cell Mol Neurobiol , 23(3):305-1, 2003. PubMed

Ostrom RS, Rana BK and Insel PA. Stoichiometry of G Protein-coupled Receptor Signaling: Implications in the Genomic Era. Pharmaceutical News, 9:295-303, 2003. pdf

Ostrom RS. New determinants of receptor-effector coupling: trafficking and compartmentation in membrane microdomains. Mol Pharmacol , 61(3):473-6, 2002. PubMed

Insel PA, Ostrom RS, Zambon AC, Hughes RJ, Balboa MA, Shehnaz D, Gregorian C, Torres B, Firestein BL, Xing M, Post SR. P2Y receptors of MDCK cells: epithelial cell regulation by extracellular nucleotides. Clin Exp Pharmacol Physiol , 28(4):351-4 , 2001. PubMed

Ostrom RS, Post SR and Insel PA. Stoichiometry and compartmentation in G protein-coupled receptor signaling: implications for therapeutic interventions involving Gs. J Pharmacol Exp Ther , 294(2):407-412, 2000. PubMed

Post SR, Ostrom RS and Insel PA. Biochemical methods for detection and measurement of cyclic AMP and adenylyl cyclase activity . Methods Mol Bio , 126:363-74 , 2000. PubMed

Ostrom RS and Insel PA. Caveolar microdomains of the sarcolemma: compartmentation of signaling molecules comes of age. Circ Res , 84(9):1110-2 , 1999. PubMed